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β-elemene reverses chemoresistance of breast cancer via regulating MDR-related microRNA expression.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Jun Zhang, He da Chen, Lin Sun, D. W. Mao, Chang Fei Chen, Wei Shone Wu, Jian Zhong Zhong, Shan Liang Zhao, Jian Hua Tang, Jin |
| Copyright Year | 2014 |
| Abstract | BACKGROUND Multidrug resistance (MDR) directly contributes to the clinical failure of chemotherapy in breast cancer (BCA). β-elemene is a natural antitumor drug from plants. We previously confirmed that MDR could be reversed by β-elemene. In this study, we intended to investigate the reversal effect of β-elemene on MDR in human BCA adriacin (Adr) -resistant MCF-7 cells (MCF-7/Adr) and docetaxel (Doc) - resistant MCF-7 cells (MCF-7/Doc) through the gene regulatory network. METHODS MTT-cytotoxic, miRNA microarray, Real-time quantitative PCR, Dual Luciferase Activity Assay, Western blot analysis were performed to investigate the impact of β-elemene on chemo-resistant BCA cell suvival, and its impact on the expression of chemo-resistance specific miRNA and the downstream target genes PTEN and Pgp. RESULTS Compared with the miRNAs expression profiles of MCF-7/Adr and MCF-7/Doc cell lines from our previous studies, there were 322 differentially expressed miRNAs in MCF-7/Adr and MCF-7/Doc breast cancer cells with β-elemene intervention (50μM/L) for 30h, and 6 miRNAs were significantly up-regulated and 12 miRNAs were significantly down-regulated in both MCF-7/Adr and MCF-7/Doc. We have testified that 5 miRNA is related to MDR before, in this study, the expression of miR-34a, miR-222, miR-452 and miR-29a can lead to changes of the characteristics of chemo-resistant MCF-7/Adr and MCF-7/Doc. The PTEN expression under intervention of β-elemene was significantly increased and Pgp expression under β-elemene intervention was significantly decreased in both cell lines. CONCLUSIONS β-elemene could influence MDR related miRNA expression and subsequently regulate the expression of the target genes PTEN and Pgp, which may lead to reduction of the viability of the chemo-resistant breast cancer cells. |
| Starting Page | 600 |
| Ending Page | 600 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.karger.com/Article/Pdf/366398 |
| PubMed reference number | 25562151v1 |
| Alternate Webpage(s) | https://doi.org/10.1159/000366398 |
| DOI | 10.1159/000366398 |
| Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology |
| Volume Number | 34 |
| Issue Number | 6 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adriacin Chemotherapy Cultured Cell Line Doxorubicin Luciferases Mammary Neoplasms MicroRNAs Multi-Drug Resistance Reverse Transcriptase Polymerase Chain Reaction Western Blot breast cancer-associated antigen 200, human cancer cell docetaxel monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
