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Triglyceride-rich lipoproteins isolated by selected-affinity anti-apolipoprotein B immunosorption from human atherosclerotic plaque.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lespine, Anne Colyvas, N. Chaumeton, A. H. Tweedie-Hardman, J. Kotite, Leila Kunitake, Steven T. |
| Copyright Year | 1994 |
| Abstract | We isolated and characterized immunoreactive apolipoprotein B (apoB)-containing lipoproteins from human atherosclerotic plaque and plasma to determine whether very-low-density lipoprotein (VLDL) can enter and become incorporated into the atherosclerotic lesion and how plaque apoB-containing lipoproteins differ from apoB-containing lipoproteins isolated from plasma. Atherosclerotic plaques were obtained during aortic surgery and processed immediately. Lipoproteins were extracted from minced plaque in a buffered saline solution (extract A). In selected cases a second extraction was done after plaque was incubated with collagenase (extract B). Lipoproteins were then isolated from the extracts by anti-apoB immunosorption and separated into VLDL + intermediate-density lipoprotein (IDL) (d < 1.019 g/mL) and low-density lipoprotein (LDL) (1.019 < d < 1.070 g/mL) fractions by ultracentrifugation. The VLDL + IDL fractions from plaque contained more than one third of the total apoB-associated lipoprotein cholesterol in both extracts A and B. The lipid composition of VLDL + IDL in both extracts was related to that of plasma VLDL + IDL. By electron microscopy mean particle diameters of VLDL + IDL from extracts A and B were 9% and 23%, respectively, greater than VLDL + IDL diameters from plasma. Mean diameters of LDL from extracts A and B were 11% and 31% greater than LDL diameters from plasma. The apoE-apoB ratio of extract A VLDL + IDL was nearly twice that of plasma VLDL + IDL and severalfold higher than that of extract A LDL. Immunoblots of both VLDL + IDL and LDL from extract A demonstrated minimal fragmentation of apoB.(ABSTRACT TRUNCATED AT 250 WORDS) |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/14/11/1767.full.pdf |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/14/11/1767.full.pdf?download=true |
| PubMed reference number | 7947602v1 |
| Volume Number | 14 |
| Issue Number | 11 |
| Journal | Arteriosclerosis and thrombosis : a journal of vascular biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Affinity Apolipoprotein E Apolipoproteins B Atherosclerosis Buffers COLLAGENASE Contain (action) Dental Plaque Diameter (qualifier value) Extraction Intermediate density lipoprotein cholesterol measurement (procedure) Lipoproteins Plaque, Atherosclerotic Saline Solution Senile Plaques Triglycerides Ultracentrifugation incubated |
| Content Type | Text |
| Resource Type | Article |
