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Microfluidic Cell Sorter Aided Live Cell Screening for Improved Fluorescent Protein
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhao, Yongxin Hoi, Hiofan Campbell, Robert E. Harrison, D. Jed |
| Copyright Year | 2012 |
| Abstract | We demonstrate a simple and low cost microFACS platform with an integrated piezoelectric actuator for directed evolution of fluorescence protein (FP) and FP-based indicators with high throughput up to 500 cells/s. The system was first employed to screen a 10 6 member library of a novel yellow FP. Compared to the manual on-plate screening method, the average brightness of the top 10 variants identified by our system was 50% brighter. The system also achieved 6-fold enrichment in isolating cells expressing superior FP-based indicator G-GECO1.0 from flash-pericam, showing the capability of screening FP-based indicators for brighter fluorescence and better functions. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/images/loc/2012/pdf/T.3.89.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
