Microfluidic Tissue Model for Live Cell Screening

Content Provider	Scilit
Author	Lee, P. J. Gaige, T. A. Ghorashian, N. Hung, P. J.
Copyright Year	2007
Description	Journal: Biotechnology Progress We have developed a microfluidic platform modeled after the physiologic microcirculation for multiplexed tissue-like culture and high-throughput analysis. Each microfabricated culture unit consisted of three functional components: a 50 microm wide cell culture pocket, an artificial endothelial barrier with 2 microm pores, and a nutrient transport channel. This configuration enabled a high density of cancer cells to be maintained for over 1 week in a solid tumor-like morphology when fed with continuous flow. The microfluidic chip contained 16 parallel units for "flow cell" based experiments where live cells were exposed to a soluble factor and analyzed via fluorescence microscopy or flow-through biochemistry. Each fluidically independent tissue unit contained approximately 500 cells fed with a continuous flow of 10 nL/min. As a demonstration, the toxicity profile of the anti-cancer drug paclitaxel was collected on HeLa cells cultured in the microfluidic format and compared with a 384-well dish for up to 5 days of continuous drug exposure.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2532848/pdf
Ending Page	951
Page Count	6
Starting Page	946
e-ISSN	15206033
DOI	10.1021/bp070053l
Journal	Biotechnology Progress
Issue Number	4
Volume Number	23
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2007-08-03
Access Restriction	Open
Subject Keyword	Journal: Biotechnology Progress
Content Type	Text
Resource Type	Article