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Conserved portion in bacterial ribosomal RNA.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Takahashi, Hideo Saito, Hiuga Ikeda, Yonosuke |
| Copyright Year | 2004 |
| Abstract | The conserved portion in bacterial ribosomal RNA was studied by the DNA-RNA hybridization method. The hybridization percentages were as follows: Bacillus subtilis DNA and B. subtilis 23S rRNA, 0.16; Escherichia coli DNA and E. coli 23S rRNA, 0.15; B. subtilis DNA and E. coli 23S rRNA, 0.03; E. coli DNA and B. subtilis 23S rRNA, 0.04. The RNA's extracted from the heterologous hybrids could be rehybridized with DNA's of B. subtilis and E. coli. The average chain lengths of the RNA's were estimated by sucrose density gradient centrifugation and Sephadex gel filtration. The results suggested that the size might be larger than 30 nucleotides. Nucleotide compositions of the RNA's in the hybrids were also studied. Both RNA's contained higher molar percentages of guanylic acid and cytidylic acid than the whole rRNA's. |
| Starting Page | 1 |
| Ending Page | 10 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.jstage.jst.go.jp/article/jgam1955/15/2/15_2_209/_pdf |
| PubMed reference number | 15965891v1 |
| Volume Number | 50 |
| Issue Number | 6 |
| Journal | The Journal of general and applied microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Centrifugation Centrifugation, Density Gradient Composition Contain (action) Cytidine Monophosphate Gel Chromatography Guanosine Monophosphate Hybrids Large Nucleic Acid Hybridization Nucleotides Ribosomal RNA Sucrose filtration |
| Content Type | Text |
| Resource Type | Article |
