Loading...
Please wait, while we are loading the content...
Similar Documents
Bioassay of Corticotrophin Using the Sheep's Transplanted Adrenal Gland.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Espiner, E. A. Beaven, Donald W. Hart, Douglas |
| Copyright Year | 1963 |
| Abstract | Measurement of minute amounts of adrenocorticotrophic hormone (ACTH) in body fluids at present depends upon biological assay. We here report a very sensitive method of ACTH assay using the transplanted gland in sheep whose ACTH secretions have been suppressed with 16\g=a\-methyl-9\g=a\-fluoro-\g=D\1 cortisol (dexamethasone). The animals used were three Merino ewes. The left adrenal had been transplanted to a vascular anastomosis with the left carotid and jugular vessels and enclosed in a skin loop 4-8 months previously, using the technique of McDonald, Goding & Wright (1958). The right adrenal was removed 2-4 weeks later. Experiments were conducted on each animal in turn at monthly intervals, when blood was collected from the adrenojugular vein before and after carotid-arterial injections of test fluid. A fluorescent technique (De Moor, Raskin & Steeno, 1960) was used to measure cortisol\p=m-\themain glucocorticoid secreted by the sheep (Bush & Ferguson, 1953)\p=m-\in2 ml. samples of adrenal effluent plasma. The rate of cortisol secretion was calculated from concentra¬ tion and adrenal plasma flow per minute. Complete suppression of adrenocortical secretion was achieved by 10-12 mg. dexamethasone given as three intramuscular injections over 18 hr. preceding the experiments. Preliminary work indicated that under these conditions the maximum response in cortisol secretion occurred 6-11 min. after beginning a 2 min. perfusion of a standard dose of ACTH. On the day of assay the adreno-jugular vein loop was catheterized and pure adrenal |
| Starting Page | 6615 |
| Ending Page | 6615 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://joe.bioscientifica.com/downloadpdf/journals/joe/27/2/joe_27_2_012.pdf |
| PubMed reference number | 14079515v1 |
| Volume Number | 27 |
| Journal | The Journal of endocrinology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adrenal Glands Biological Assay Blood Vessel Body Fluids Catheterization Corticotropin Dexamethasone Epinephrine Hydrocortisone Liquid substance Transplantation cortisol secretion |
| Content Type | Text |
| Resource Type | Article |
