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Comparison of multiplex PCR assay with culture for detection of genital mycoplasmas.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Stellrecht, Kathleen A. Woron, Amy M. Mishrik, Nada G. Venezia, R. |
| Copyright Year | 2004 |
| Abstract | Ureaplasma, spp. Mycoplasma genitalium, and Mycoplasma hominis are associated with infection of the genitourinary tract, reproductive failure, and neonatal morbidity and mortality. We have developed a multiplex PCR for the detection of these Mycoplasmatales in a single amplification reaction. The analytical sensitivities of this assay were 10.8, 10.8, and 8.8 CFU for each organism, respectively. This multiplex PCR was compared to culture on 26 cervical swabs, 2 vaginal swabs, 4 female urine specimens, 49 semen samples, 2 male urine specimens, and 1 nonspecified sample. A total of 21 specimens were culture positive (25%); 17 of these were PCR positive. An additional 11 specimens were PCR positive but culture negative. Of the 21 culture-positive specimens, 17 (81%) grew Ureaplasma spp. and 4 (19%) grew Mycoplasma spp. Of the 28 PCR-positive specimens, Ureaplasma spp. was detected in 23 (82%), M. hominis was detected in 3 (11%), and both were detected in 2 (7%). In a confirmatory analysis, all samples were tested by amplification of a second target of the ureaplasma genome. True-positive cases were defined as a positive result by culture or by both amplification assays. The multiplex PCR detected organisms in 26 of the 30 true-positive specimens, as well as in 2 other specimens. Based on a 36% prevalence of infection, the sensitivity, specificity, and positive and negative predictive values of multiplex PCR analyses were 87, 96, 94, and 93%, respectively. Multiplex PCR offers a rapid, sensitive, and easy method to detect genital mycoplasmas. |
| Starting Page | 67 |
| Ending Page | 74 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcm.asm.org/content/42/4/1528.full.pdf |
| PubMed reference number | 15070999v1 |
| Volume Number | 42 |
| Issue Number | 4 |
| Journal | Journal of clinical microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Arthrogryposis Colony-forming unit Forty Nine Genitourinary system Morbidity - disease rate Multiplex Polymerase Chain Reaction Mycoplasma genitalium Mycoplasmatales (bacterium) Neck Specimen Steatocystoma multiplex Swab Ureaplasma Vagina hominy |
| Content Type | Text |
| Resource Type | Article |
