NDLI: Rapid detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms in genitourinary samples by PCR-microtiter plate hybridization assay.

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Rapid detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms in genitourinary samples by PCR-microtiter plate hybridization assay.

Content Provider	Semantic Scholar
Author	Yoshida, Takashi Kariya Shin-Ichi Deguchi, Takashi Miyazawa, Takamaro Ishiko, Hiroaki
Copyright Year	2003
Abstract	We present a method for detecting the presence of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms, which are thought to be associated with nongonococcal urethritis (NGU) and other genitourinary infections, in clinical samples. This method consists of PCR amplification of a part of the 16S rRNA gene followed by 96-well microtiter plate hybridization assay using four species-specific capture probes to detect the targets. To test the efficacy of this method, we applied it to the detection of the four species in the urine of patients with NGU. There were no cross-reactions with other human mycoplasmas or ureaplasmas, and the PCR-microtiter plate hybridization assay detected as few as 10 copies of the 16S rRNA gene of each of the four species. Based on these results, this PCR-microtiter plate hybridization assay can be considered an effective tool for the diagnosis of genitourinary infections with mycoplasmas or ureaplasmas.
File Format	PDF HTM / HTML
DOI	10.1128/JCM.41.5.1850-1855.2003
PubMed reference number	12734216
Journal	Medline
Volume Number	41
Issue Number	5
Alternate Webpage(s)	http://jcm.asm.org/content/41/5/1850.full.pdf
Alternate Webpage(s)	https://doi.org/10.1128/JCM.41.5.1850-1855.2003
Journal	Journal of clinical microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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