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Effets de plantes réputées antidiabétiques sur un modèle cellulaire hépatique de résistance à l’insuline induite par le palmitate
| Content Provider | Semantic Scholar |
|---|---|
| Author | Afshar, Arvind |
| Copyright Year | 2011 |
| Abstract | Cree pharmacopeia is rich in medicinal plants and many of them are studied by our laboratory for their antidiabetic potential. Some of the species tested have shown to activate the AMP-activated protein kinase (AMPK), an enzyme responsible for the translocation of glucose transporters to the cell membrane (hypoglycaemic activity). AMPK is also known to activate other cellular functions, like fat oxidation, in order to restore cell energy loss. The objectives of this study are, first, to measure the metabolic stress induced by eight of the species in muscular and liver cells, an effect believed to be responsible for the AMPK activation. Metabolic stress is evaluated by measuring extracellular medium acidification and cellular ATP depletion. The second objective is to assess the capacity of the extracts to clear intracellular fat (fat oxidation) and, by doing this, restore insulin sensitivity in insulinresistant driven hepatocytes. To become insulin-resistant (a condition strongly linked to obesity), the hepatocytes are treated with a saturated fatty acid, palmitate. The results show that most of the extracts seem to increase the metabolic stress in muscular cells and hepatocytes for a short period of time. Among all extracts, only one has significantly reduced intracellular triglycerides in palmitate treated hepatocytes, an effect not followed by an increase in insulin sensitivity. In conclusion, the species tested in this study seem to exert their hypoglycaemic potential by affecting mitochondrial respiration (metabolic stress). However, the experimentations have not clearly shown the capacity of the species to restore insulin sensitivity in insulin-resistant liver cells. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://papyrus.bib.umontreal.ca/xmlui/bitstream/handle/1866/12751/Afshar_Arvind_2010_memoire.pdf?isAllowed=y&sequence=8 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
