NDLI: Carbapenems as inhibitors of OXA-13, a novel, integron-encoded beta-lactamase in Pseudomonas aeruginosa.

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Carbapenems as inhibitors of OXA-13, a novel, integron-encoded beta-lactamase in Pseudomonas aeruginosa.

Content Provider	Semantic Scholar
Author	Mugnier, Pauline D. Podglajen, Isabelle Goldstein, Fred W. Collatz, Ekkehard
Copyright Year	1998
Abstract	A clinical Pseudomonas aeruginosa strain, PAe391, was found to be resistant to a number of antibiotics including ticarcillin, piperacillin, cefsulodin and amikacin, and a disk diffusion assay showed evidence of pronounced synergy between imipenem and various beta-lactam antibiotics. Cloning and nucleotide sequence analysis revealed the dicistronic arrangement of an aac(6')-Ib variant and a novel blaOXA-type gene between the intI and qacE delta 1 genes typical of integrons, in PAe391, this integron was apparently chromosome-borne. The beta-lactamase, named OXA-13, displayed nine amino acid changes with respect to OXA-10:I in position 10 of OXA-10 to T (I10T), G20S, D55N, N73S, T107S, Y174F, E229G, S245N and E259A, OXA-13 (pIapp = 8.0) showed poor catalytic activity against penicillins as well as cephalosporins, but was efficient in hydrolysing some penicillinase-resistant beta-lactams, such as cefotaxime and aztreonam. It was efficiently inhibited by imipenem (KIapp = 11 nM), and formed a stable complex. While the KIapp value of meropenem was similar (16 nM), the corresponding complex was less stable.
Starting Page	5790
Ending Page	5793
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://mic.sgmjournals.org/content/144/4/1021.full.pdf
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/micro/144/4/mic-144-4-1021.pdf?accname=guest&checksum=55DCD44E6045C51D67A9DDE128EB512C&expires=1544576667&id=id
PubMed reference number	9579076v1
Volume Number	144
Part	4
Journal	Microbiology
Language	English
Access Restriction	Open
Subject Keyword	Amikacin Amino Acids Aztreonam Base Sequence Carbapenems Cefotaxime Cefsulodin Cephalosporins Imipenem Integrons Lactams Monobactams Name Nucleotides Penicillinase Penicillins Piperacillin Pseudomonas aeruginosa Sequence Analysis Ticarcillin beta-Lactamase beta-Lactams meropenem
Content Type	Text
Resource Type	Article

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