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HIGH-THROUGHPUT CIRCULATING TUMOR CELLS (CTCs) ISOLATION USING INERTIAL FORCES
Content Provider | Semantic Scholar |
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Author | Bhagat, Shekhar Huang, Sha Lim, Chwee Teck Han, Jongyoon |
Copyright Year | 2010 |
Abstract | Continuous flow–based cell separations are indispensable for many biomedical applications and diagnostics including early detection of cancer. Here, we describe a high-throughput cell separation technique for sorting circulating tumor cells (CTCs) from blood using microfluidics. The separation consists of a 2-step process to efficiently isolate CTCs from blood. In the first step, 99% of hematologic cells (erythrocytes (RBCs) and leukocytes) are removed using shearmodulated inertial focusing with >90% CTC recovery. This RBCs depleted sample is then run through a low aspect ratio spiral microchannel to completely isolate the CTCs. The operating principle of this separation method exploits the difference in cell size between CTCs (~16-20 µm diameter) and other blood cells (RBCs ~8 µm; leukocytes ~8-14 µm). |
File Format | PDF HTM / HTML |
Alternate Webpage(s) | http://www.rsc.org/binaries/LOC/2010/PDFs/Papers/474_0181.pdf |
Language | English |
Access Restriction | Open |
Content Type | Text |
Resource Type | Article |