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Anticancer Activity of Phloretin Against Human Gastric Cancer Cell Lines Involves Apoptosis, Cell Cycle Arrest, and Inhibition of Cell Invasion and JNK Signalling Pathway
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xu, Min Gu, Weiguang Shen, Zhou Qiang Wang, Fenghua |
| Copyright Year | 2018 |
| Abstract | BACKGROUND Gastric cancer is one of most commonly diagnosed cancers and causes significant mortality worldwide. In this study, the antiproliferative and anticancer effects of Phloretin were evaluated against gastric cancer cell lines. MATERIAL AND METHODS MTT assay was used to assess the proliferation rate of gastric cancer cells. DAPI and annexin V/PI were used for detection of apoptotic cell death. Cell invasion was investigated by Transwell assays and the expression of the proteins was estimated by immunoblotting. RESULTS The results revealed that Phloretin exerts anticancer effects on all the gastric cancer cell lines used in this study. However, the anticancer effects were more significant (p<0.05) on the AGS cell line. Further, the effect of Phloretin on the viability of normal GES-1 cells was minimal. Apoptosis assays showed that Phloretin triggers apoptotic cell death in AGS gastric cancer cells. Phloretin could also cause the arrest of the AGS gastric cancer cells in the G2/M phase of the cell cycle and suppress their ability to migrate. Western blotting analysis revealed that Phloretin significantly decreased the expression of p-JNK and p-38. However, comparatively lower effects were observed on the expression of JNK and P38. CONCLUSIONS We showed that Phloretin is an important molecule for the treatment of gastric cancer. |
| Starting Page | 6551 |
| Ending Page | 6558 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 30224626v1 |
| Alternate Webpage(s) | https://doi.org/10.12659/MSM.910542 |
| DOI | 10.12659/msm.910542 |
| Journal | Medical science monitor : international medical journal of experimental and clinical research |
| Volume Number | 24 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | AICARDI-GOUTIERES SYNDROME 1 Annexins Apoptosis Cell Cycle Arrest Cell Death Cultured Cell Line Immunoblotting Leukemia, B-Cell Malignant Neoplasms Malignant neoplasm of stomach Mitosis Non-Small Cell Lung Carcinoma Phloretin Positive Regulation of Apoptosis Precipitating Factors Stomach Carcinoma Western Blotting cancer cell monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
