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The effect of leptin and resveratrol on JAK/STAT pathways and Sirt-1 gene expression in the renal tissue of ischemia/reperfusion induced rats.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Erkasap, Serdar Erkasap, Nilufer Bradford, Baxter Mamedova, Liaman Uysal, Onur Ozkurt, Mete Ozyurt, Refik Kutlay, Ozden Bayram, Banu |
| Copyright Year | 2017 |
| Abstract | OBJECTIVE Our study aimed to investigate the possible modifying effects of leptin and combined use of resveratrol on rat renal I/R injury and their relationship on signal pathways and apoptosis-related mechanisms. BACKGROUND Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure. METHODS Male Sprague Dawley rats were divided into 5 groups: Control, I/R, I/R+leptin, I/R+resveratrol and I/R+leptin+resveratrol. Leptin (10 μg/kg BW) was administered (i.p.) 30 min prior to I/R. Resveratrol was administered by gavage at 20 mg/kg BW per d for 12 d prior to I/R. The left renal artery was exposed to 1 h of ischemia and 1 h of reperfusion. RESULTS Resveratrol treatment alone increased TNF-α, TNF-α R1, NF-κB, SIRT-1, STAT1 and STAT3 mRNA levels and decreased caspase 3 protein levels. Leptin treatment alone significantly decreased the caspase 3 protein levels. The combined use of resveratrol and leptin significantly increased STAT3, and caspase 3 mRNA levels, and decreased the caspase 3 protein levels. Apoptosis was significantly decreased especially in the leptin and leptin+resveratrol groups. CONCLUSION The present study suggest that a combined use of resveratrol and leptin has preventive and regulatory effects on renal I/R injury; the mechanism involves decreasing apoptosis, likely by altering the JAK/STAT pathway and SIRT1 expression (Fig. 8, Ref. 24). |
| Starting Page | 443 |
| Ending Page | 448 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.4149/BLL_2017_086 |
| PubMed reference number | 29050480 |
| Journal | Medline |
| Volume Number | 118 |
| Issue Number | 8 |
| Alternate Webpage(s) | http://www.elis.sk/download_file.php?product_id=5325&session_id=j50bpb6jdfvup4vu877dase064 |
| Alternate Webpage(s) | https://doi.org/10.4149/BLL_2017_086 |
| Journal | Bratislavske lekarske listy |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
