Loading...
Please wait, while we are loading the content...
Similar Documents
The in vitro protective effect of PNS in myocardial reperfusion injuries mediated through the JAK 2 / STAT 3-SIRT 1 signaling pathways
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wu, Jiajun Zhang, Zhiling Wang, Fanlin Yang, Tianye |
| Copyright Year | 2019 |
| Abstract | Background: The present study aimed to investigate the function of the JAK2/STAT3 and the SIRT1 signaling pathways in the normal myocardium, and the protective effect of PNS in myocardial ischemia injury. Material and methods: JAK2 siRNA was transfected, and the MTT assay was applied to detect the cardiomyocytes’ viability, and Western blotting was employed to measure the protein expressions. Following this, the impacts of JAK2 siRNA, STAT3 siRNA and SIRT1 siRNA SIRI on the protective effects of PNS on the expressions of p-JAK2, JAK2, p-STAT3, STAT3, SIRT1, and Ac-FOXO1 in SIRI cardiomyocytes were measured to explore the role of PNS within. Result: The experiment found that, in the normal myocardial cell model, JAK2 siRNA, STAT3 siRNA and SIRT1 siRNA had no significant effect on myocardial viability. JAK2 siRNA and STAT3 siRNA can inhibit both the JAK2/STAT3 and SIRT1 pathways. SIRT1 siRNA can inhibit the SIRT1 pathway and reduce p-JAK2 and p-STAT3 expressions. In the PNSadministered SIRI cardiomyocyte model, JAK2 siRNA, STAT3 siRNA and SIRT1 siRNA can reverse the protective effects of PNS on cardiomyocytes, through which PNS could significantly increase the p-JAK2, p-STAT3 and SIRT1 expressions. Both the JAK2/STAT3 and SIRT1 pathways activated by PNS were reversed by JAK2 siRNA and STAT3 siRNA, and likewise in the JAK2/STAT3 pathway, the p-JAK2 and p-STAT3 expressions decreased, while in the SIRT1 pathway, SIRT1 decreased and Ac-FOXO1 increased. Moreover, SIRT1 siRNA can reverse the SIRT1 pathway activated by PNS, in which SIRT1 decreased, Ac-FOXO1 increased, and the JAK2/STAT3 pathway was also affected as the p-JAK2 and p-STAT3 expressions increased. Conclusions: In the normal cardiomyocyte model, JAK2 siRNA, STAT3 siRNA, and SIRT1 siRNA have no significant effects on myocardial viability but can reverse the protective effects of PNS on cardiomyocytes. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ijcem.com/files/ijcem0073741.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
