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Photolysis-induced suppression of inhibition in rat hippocampal CA1 pyramidal neurons.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Jordon K. Zucker, Robert S. |
| Copyright Year | 2001 |
| Abstract | 1. Whole cell patch clamp recording, Ca(2+) measurement with ratiometric fluorescent dyes and photolysis of caged Ca(2+) were combined to investigate the depolarization- and photolysis-induced suppression of inhibition (DSI and PSI) in rat hippocampal CA1 pyramidal cells. 2. A 5-s depolarization from -70 mV to 0 mV or a 6-s photolysis of nitrophenyl-EGTA (NPE) in cell bodies could each depress the frequency of spontaneous inhibitory postsynaptic currents (IPSCs) and the amplitude of evoked IPSCs while elevating intracellular Ca(2+) concentration ([Ca(2+)](i)). 3. Within a cell the elevation of [Ca(2+)](i) induced by depolarization was inversely related to that induced by photolysis, suggesting that higher [NPE] is more effective in releasing caged Ca(2+) but also increases buffer capacity to reduce [Ca(2+)](i) rises caused by Ca(2+) influx through voltage-dependent Ca(2+) channels. 4. Both DSI and PSI were linearly related to [Ca(2+)](i), with a 50 % reduction in transmission occurring at about 3.6--3.9 microM. 5. [Ca(2+)](i) recovered more quickly than DSI, indicating that the duration of DSI is not set simply by the duration of [Ca(2+)](i) elevation, but rather entails other rate-limiting processes. 6. We conclude that DSI is activated by micromolar [Ca(2+)](i) acting far from sites of Ca(2+) entry through channels in the plasma membrane. |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1469-7793.2001.t01-1-00757.x |
| PubMed reference number | 11410632 |
| Journal | Medline |
| Volume Number | 533 |
| Part | 3 |
| Alternate Webpage(s) | http://mcb.berkeley.edu/labs/zucker/PDFs/Wang_JPhy533,757.pdf |
| Alternate Webpage(s) | https://mcb.berkeley.edu/labs/zucker/PDFs/Wang_JPhy533,757.pdf |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1469-7793.2001.t01-1-00757.x |
| Journal | The Journal of physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
