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Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: contrast to the tetrameric Pseudomonas counterpart.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yonezawa, Yasushi Izutsu, Ken-Ichi Tokunaga, Hiroko Hirotaka Arakawa, Tsutomu Tokunaga, Masao |
| Copyright Year | 2007 |
| Abstract | Light scattering and chemical cross-linking analyses of nucleoside diphosphate kinase (NDK) from moderate halophile, Halomonas sp. 593 (HaNDK), unambiguously demonstrated that this enzyme formed a dimeric structure, in contrast to the Pseudomonas NDK (PaNDK), a nonhalophilic counterpart, and other NDKs from Gram-negative bacteria, which all formed a tetrameric structure. Comparison of HaNDK and PaNDK showed that the HaNDK was less thermally stable than the PaNDK: the optimum temperature of PaNDK enzyme activity was 20 degrees C higher than that of HaNDK. However, the HaNDK readily refolded and reassembled back to the active dimeric structure, upon heat denaturation at 0.2 M NaCl, as soon as the temperature was lowered. On the contrary, the thermally more stable PaNDK was irreversibly denatured at its melting temperature. |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1574-6968.2007.00626.x |
| PubMed reference number | 17227453 |
| Journal | Medline |
| Volume Number | 268 |
| Issue Number | 1 |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/femsle/268/1/10.1111/j.1574-6968.2007.00626.x/2/268-1-52.pdf?Expires=1497943390&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=bGp6l8hWm~Bq1ZriTt6ZfFE7euUOTqg63QSwGTxn0p8sOyuxzLxv7GYXbx6sjl8IDJc4boC8CnCklm7tsltVh3WJBaY2tGWU5vR2MS8~ic579P0Y1HGqrAqr-pVK5z6n9oc577dm21Kuslrol7NcAxQpIRa51Hqx3yZR1CerYke9x-TnX~JTjrHt8yPKLAvWq~OVUPeDLFzcZ4mJrNXfb4o~58AbJ2gqmbWq5MROAV2wqRDZ-acz31J3jw-fz1WiCo1CLe1fQ-Podimp9C63Y2ukJwkrtjtLs0-qzrcDTG027SZWM5K~NC5msMhHdi1H9gikXZyQYhxV8ufYWLgnRw__ |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1574-6968.2007.00626.x |
| Journal | FEMS microbiology letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |