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Comparación de la sensibilidad analítica de métodos de tinción tradicionales en la detección del protozoario zoonótico Cryptosporidium parvum en muestras de agua inoculadas con el agente
| Content Provider | Semantic Scholar |
|---|---|
| Author | Obregon, Roberto |
| Copyright Year | 2010 |
| Abstract | Cryptosporidiosis is a parasitic disease of the digestive tract caused by protozoa Cryptosporidium spp. Although the species of this agent have been reported since the beginning of the twentieth century, at the end of it has been recognized as a pathogen widely distributed in different animals and a threat to public health. This protozoan has been described in all continents, including Antarctica. The infection in humans and some animals is considered a reemerging zoonosis. The first outbreak of cryptosporidiosis transmitted through the water was described in 1984, since then many authors have shown the presence of this endoparasite in surface and untreated waste water and its role as the etiologic agent of diarrhea. The persistence and spread of Cryptosporidium in the environment it is based on three biological events: (1) Its oocysts are immediately infectious, (2) resist environmental conditions and (3) resist conventional disinfectants and was not affected by the physical processes of water treatment, it can survive for several months. Although there are various methodologies described in the world, to detect the parasite in samples of this type, this study would be the first attempt to develop a standardized protocol, so far in Chile. Therefore, the main objective of this study was to compare the sensitivity of the Ziehl-Neelsen and Aureamina staining methods for the recovery, concentration and detection of human and bovine Cryptosporidium oocysts of in water samples in Chile. For this purpose, oocysts of C. parvum isolated from feces of diarrheic calves and humans, previously studied by Crypto-Strip ® (immunechromatography) and species-specific PCR, were artificially inoculated in water samples in decreasing concentrations. The lowest number of Cryptosporidium oocysts that are required to give a positive water sample was used for analytical sensitivity comparison of both staining methods. The results, expressed as (oocysts/mL), showed that both staining methods have similar analytical sensitivity, as they are able to detect 1,953 Cryptosporidium oocysts in 250 mL of water, ie, 7,8 oocysts/mL. Concluding, the selection of either modified Ziehl-Neelsen or Aureamina staining methods to detect this parasite in water samples, should not be based in the sensitivity of the procedures, but in the methodological advantages and disadvantages for its implementation. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorio.uchile.cl/bitstream/handle/2250/131324/Comparacion-de-la-sensibilidad-analitica-de-metodos-de-tincion-tradicionales-en-la-deteccion-del-protozoario-zoonotico-Cryptosporidium-parvum-en-muestras-de-agua-inoculadas-con-el-agente.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
