COMPARACIÓN DEL LÍMITE DE DETECCIÓN DE MÉTODOS DE ANÁLISIS DE ÁCIDOS NUCLEICOS PARA EL VIROIDE DEL ENANISMO DEL LÚPULO (HSVd) EN CÍTRICOS

Content Provider	Semantic Scholar
Author	Soto, Marianela Gonzalez, Lien Ta Peralta, Esther Lilia Duran-Vila, Núria
Copyright Year	2007
Abstract	The detection limit of sequence polyacrilamide gel electrophoresis (sPAGE), nucleic acid hybridization (NASH) and reverse transcription-polymerase chain reaction (RT-PCR) was determined for the diagnosis of the hop stunt viroid (HSVd) in citrus hosts, using citron and cucumber plants as biological amplifiers. The detection limit of sPAGE was higher (5x10 -3 ) when cucumber plants were analyzed than that using citron plants (5x10 -2 ); however, it was 50 times less sensitive than that of NASH (10 -4 ) by comparison. The RT-PCR detection limit for cucumber host was the highest (10 -6 ) of all methods analyzed. This value was higher when the RT-PCR products were detected by NASH and no with agarose gel electrophoresis instead. RT-PCR was the most sensitive method and its sensitivity was increased when it was combined with NASH. However, the presence of contamination s associated with the high sensitivity of RT-PCR is a routine diagnosis problem. On the other hand, NASH is a method with a proper sensitivity under the conditions analyzed since the detection limit was higher than that of sPAGE, although slower than that of RT-PCR.
Starting Page	97
Ending Page	103
Page Count	7
File Format	PDF HTM / HTML
Volume Number	22
Alternate Webpage(s)	http://scielo.sld.cu/pdf/rpv/v22n2/rpv05207.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article