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Virus infection of Haptolina ericina and Phaeocystis pouchetii implicates evolutionary conservation of programmed cell death induction in marine haptophyte–virus interactions
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ray, Jessica Louise Haramaty, Liti Thyrhaug, Runar Fredricks, Helen F. Mooy, Benjamin A. S. Van Larsen, Aud Bidle, Kay D. Sandaa, R. |
| Copyright Year | 2014 |
| Abstract | The mechanisms by which phytoplankton cope with stressors in the marine environment are neither fully characterized nor understood. As viruses are the most abundant entities in the global ocean and represent a strong top-down regulator of phytoplankton abundance and diversity, we sought to characterize the cellular response of two marine haptophytes to virus infection in order to gain more knowledge about the nature and diversity of microalgal responses to this chronic biotic stressor. We infected laboratory cultures of the haptophytes Haptolina ericina and Phaeocystis pouchetii with CeV-01B or PpV-01B dsDNA viruses, respectively, and assessed the extent to which host cellular responses resemble programmed cell death (PCD) through the activation of diagnostic molecular and biochemical markers. Pronounced DNA fragmentation and activation of cysteine aspartate-specific proteases (caspases) were only detected in virus-infected cultures of these phytoplankton. Inhibition of host caspase activity by addition of the pan-caspase inhibitor z-VAD-fmk did not impair virus production in either host-virus system, differentiating it from the Emiliania huxleyi-Coccolithovirus model of haptophyte-virus interactions. Nonetheless, our findings point to a general conservation of PCD-like activation during virus infection in ecologically diverse haptophytes, with the subtle heterogeneity of cell death biochemical responses possibly exerting differential regulation on phytoplankton abundance and diversity. |
| Starting Page | 943 |
| Ending Page | 955 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://darchive.mblwhoilibrary.org/bitstream/handle/1912/6616/J.%20Plankton%20Res.-2014-Ray-plankt_fbu029.pdf;jsessionid=FDB3FA3D1A75037D996082B8B6BAD747?sequence=1 |
| PubMed reference number | 25013242v1 |
| Volume Number | 36 |
| Journal | Journal of plankton research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Aspartic Acid Caspase Inhibitors DNA Haptolina cf. herdlensis Haptophyta Murine sarcoma viruses Phytoplankton Positive Regulation of Cell Death VAD I protocol benzyloxycarbonyl-Phe-Ala-fluormethylketone |
| Content Type | Text |
| Resource Type | Article |
