NDLI: Effect of oxygen concentration on free radical-induced cytotoxicity.

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Effect of oxygen concentration on free radical-induced cytotoxicity.

Content Provider	Semantic Scholar
Author	Chen, Zhihua Saito, Yoshiro Yoshida, Yasukazu Niki, Etsuo
Copyright Year	2008
Abstract	Free radicals induce oxidative stress in vivo, leading to various disorders and diseases. In the present study, the effect of oxygen pressure on the cytotoxicity induced by free radicals was studied. It was found that alkyl radicals markedly aggravated Jurkat cell apoptosis under low oxygen pressure and this was ascribed to a hypoxic condition caused by the consumption of oxygen by alkyl radicals giving peroxyl radicals and subsequent lipid peroxidation by a chain mechanism. The intracellular lipid hydroperoxides significantly increased at an early time point even under hypoxia. Cytochrome c was released from the mitochondria, and caspase-9 as well as caspase-3 was activated during apoptosis, indicating that cell death followed by the intrinsic, mitochondrial apoptosis pathway. Pretreatment with VAD-FMK, a caspase inhibitor, attenuated the apoptosis induced by alkyl radicals under hypoxia. Moreover, pretreatment with various antioxidants also significantly rescued the cells from apoptosis. Taken together, the results indicate that free radicals induced hypoxic conditions, which accelerated mitochondria-dependent cell apoptosis.
Starting Page	1
Ending Page	672
Page Count	672
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/bbb/72/6/72_80002/_pdf
PubMed reference number	18540111v1
Volume Number	72
Issue Number	6
Journal	Bioscience, biotechnology, and biochemistry
Language	English
Access Restriction	Open
Subject Keyword	Antioxidants Apoptosis Caspase Inhibitors Cell Death Free Radicals Hypoxia Lipid Peroxidation Oxidative Stress Oxygen Peroxyl alkyl benzyloxycarbonyl-Phe-Ala-fluormethylketone caspase-3 caspase-9
Content Type	Text
Resource Type	Article

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