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A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Perrod, Severine Cockell, Moira Laroche, Thierry Renauld, Hubert Ducrest, A. L. Bonnard, Carine Gasser, Susan M. |
| Copyright Year | 2001 |
| Abstract | In budding yeast, the silent information regulator Sir2p is a nuclear NAD-dependent deacetylase that is essential for both telomeric and rDNA silencing. All eukaryotic species examined to date have multiple homologues of Sir two (HSTs), which share a highly conserved globular core domain. Here we report that yeast Hst2p and a mammalian Hst2p homologue, hSirT2p, are cytoplasmic in yeast and human cells, in contrast to yHst1p and ySir2p which are exclusively nuclear. Although yHst2p cannot restore silencing in a sir2 deletion, overexpression of yHst2p influences nuclear silencing events in a SIR2 strain, derepressing subtelomeric silencing while increasing repression in the rDNA. In contrast, a form of ySir2p carrying a point mutation in the conserved core domain disrupts both telomeric position effect (TPE) and rDNA repression at low expression levels. This argues that non-nuclear yHst2p can compete for a substrate or ligand specifically required for telomeric, and not rDNA repression. |
| Starting Page | 197 |
| Ending Page | 209 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://emboj.embopress.org/content/embojnl/20/1-2/197.full.pdf |
| PubMed reference number | 11226170v1 |
| Volume Number | 20 |
| Issue Number | 1-2 |
| Journal | The EMBO journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Deletion Mutation Homologous Gene Mammals NAD-dependent histone deacetylase activity Nicotinamide adenine dinucleotide (NAD) Point Mutation Repression, Psychology Saccharomyces cerevisiae chromatin silencing at telomere |
| Content Type | Text |
| Resource Type | Article |
