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Nuclear export modulates the cytoplasmic Sir2 homologue Hst2.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wilson, Jeanne Le, Viet Q. Zimmerman, Collin Marmorstein, Ronen Pillus, Lorraine |
| Copyright Year | 2006 |
| Abstract | Modulating transcription factors is crucial to executing sophisticated gene expression programs. The silent information regulator 2 (Sir2) family of NAD-dependent protein deacetylases influences transcription by targeting proteins such as histones, p53 and forkhead-box family transcription factors. Although apparently cytoplasmic, both mammalian SIRT2 and its yeast orthologue Hst2 have been implicated in transcriptional regulation. Here, we show that Hst2 moves between the nucleus and cytoplasm, but is largely cytoplasmic owing to efficient nuclear export. This nuclear exclusion is mediated by the exportin chromosomal region maintenance 1 (Crm1) and a putative leucine-rich nuclear export sequence in Hst2, which overlaps a unique autoregulatory helix. Disruption of Hst2 export shows that nuclear exclusion inhibits the activity of Hst2 as a transcriptional repressor. Our identification of putative nuclear export sequences in numerous vertebrate SIRT2 proteins shows that active nuclear export can be a conserved mechanism for regulating Sir2 homologues. |
| Starting Page | 611 |
| Ending Page | 616 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://embor.embopress.org/content/embor/7/12/1247.full.pdf |
| PubMed reference number | 17110954v1 |
| Volume Number | 7 |
| Issue Number | 12 |
| Journal | EMBO reports |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus Exclusion Gene Expression Histones Homologous Gene Leucine Mammals NAD-Dependent Deacetylase Sirtuin-2 NAD-dependent histone deacetylase activity Nicotinamide adenine dinucleotide (NAD) Nuclear Export Region of chromosome SIRT2 gene SMRT protein TRANSCRIPTION FACTOR Transcription Repressor/Corepressor Transcription, Genetic Transcriptional Regulation executing - QueryStatusCode |
| Content Type | Text |
| Resource Type | Article |
