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Calcium-dependent release of d-[3H]aspartate evoked by selective electrical stimulation of excitatory afferent fibres to hippocampal pyramidal cells in vitro
| Content Provider | Semantic Scholar |
|---|---|
| Author | Malthe-Sørenssen, D. Skrede, K. K. Fonnum, Frode |
| Copyright Year | 1979 |
| Abstract | Abstract Selective electrical stimulation of CA1 excitatory afferent fibres in the stratum radiatum of a transverse slice of the hippocampus released d -[ 3 H]aspartate in a stimulus dependent manner, d -aspartate being used to mimic endogenous l -glutamate. The release fulfilled several of the requirements for neurotransmitter identification in as much as it was Ca 2+ -dependent and specific. Neither [ 3 H]γ-aminobutyrate nor l -[ 3 H]leucine were released in response to stimulation. The stimulus dependent release of d -[ 3 H]aspartate could also be demonstrated in an isolated preparation of the hippocampal slice containing the CA3/CA1 region, but devoid of the area dentata and subiculum. A cut carefully placed in the isolated CA3/CA1 slice dividing all CA1 afferents coursing through the stratum radiatum, without sectioning the pyramidal layer nor the stratum oriens/alveus, abolished the stimulus-evoked release of d -[ 3 H]aspartate. The results, together with previous work, favour l -glutamate as the neurotransmitter of the Schaffer collaterals of the CA3 pyramidal cells. |
| Starting Page | 1255 |
| Ending Page | 1263 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0306-4522(79)90155-6 |
| PubMed reference number | 492535 |
| Journal | Medline |
| Volume Number | 4 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0306452279901556 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0306452279901556?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0306-4522%2879%2990155-6 |
| Journal | Neuroscience |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
