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Calcium-dependent inactivation of the monosynaptic NMDA EPSCs in rat hippocampal neurons in culture.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Medina, Igor Leinekugel, Xavier Ben-Ari, Yehezkel |
| Copyright Year | 1999 |
| Abstract | The effects of increased dendritic calcium concentration ([Ca2+]i) induced by single action potentials on monosynaptic glutamatergic excitatory postsynaptic currents (EPSCs) were studied in cultured rat hippocampal neurons. To investigate the respective roles of pre- and postsynaptic elements in the depolarization-induced NMDAR inactivation, we have performed simultaneous paired whole-cell recordings from monosynaptically connected pre- and postsynaptic hippocampal neurons. We report that the single firing of the postsynaptic neuron did not result in inactivation of the NMDAR-EPSC, whereas a burst of depolarizing steps transiently depressed the NMDAR-EPSCs in both pyramidal cells and interneurons. This effect was mediated by postsynaptic voltage-gated Ca2+ influx, as it was prevented by: (i) buffering postsynaptic [Ca2+]i with 30 mM BAPTA; (ii) removing extracellular Ca2+; or (iii) applying Cd2+o (100 microM), a voltage-gated calcium channel blocker. It does not involve presynaptic mechanisms as it selectively affected NMDA but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor-mediated EPSCs. These results suggest that inactivation of NMDAR-channels by voltage-gated Ca influx is a general property of hippocampal neurons, which may play an important role in reducing postsynaptic NMDAR Ca2+ influx that leads to plasticity or excitotoxicity during sustained neuronal activity. |
| Starting Page | 1 |
| Ending Page | 5 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://pdfs.semanticscholar.org/3c3c/9d3a0e0a7b4822fa8c2aad46725d9e1c0cd8.pdf |
| Alternate Webpage(s) | http://www.inmed.univ-mrs.fr/maj/upload/publications/MedinaEurJNeurosci1999-2.pdf |
| PubMed reference number | 10383632v1 |
| Volume Number | 11 |
| Issue Number | 7 |
| Journal | The European journal of neuroscience |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Action Potentials Calcium Channel Blockers Calcium ion Depressive disorder Excitatory Postsynaptic Currents Hippocampus (Brain) Interneurons N-Methyl-D-Aspartate Receptors N-Methylaspartate Neurons Pyramidal Cells type I interferon receptor |
| Content Type | Text |
| Resource Type | Article |
