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The Impact of NOD2 Variants on Fecal Microbiota in Crohn’s Disease and Controls Without Gastrointestinal Disease
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kennedy, N. A. Lamb, Christopher Andrew Berry, Susan H. Walker, Alan W. Mansfield, John C. Parkes, Miles Simpkins, Rachel C. Tremelling, M. J. Nutland, Sarah L. Parkhill, Julian Probert, Christopher Sj Hold, Georgina L. Lees, Charlie W. |
| Copyright Year | 2018 |
| Abstract | Background/Aims Current models of Crohn's disease (CD) describe an inappropriate immune response to gut microbiota in genetically susceptible individuals. NOD2 variants are strongly associated with development of CD, and NOD2 is part of the innate immune response to bacteria. This study aimed to identify differences in fecal microbiota in CD patients and non-IBD controls stratified by NOD2 genotype. Methods Patients with CD and non-IBD controls of known NOD2 genotype were identified from patients in previous UK IBD genetics studies and the Cambridge bioresource (genotyped/phenotyped volunteers). Individuals with known CD-associated NOD2 mutations were matched to those with wild-type genotype. We obtained fecal samples from patients in clinical remission with low fecal calprotectin (<250 µg/g) and controls without gastrointestinal disease. After extracting DNA, the V1-2 region of 16S rRNA genes were polymerase chain reaction (PCR)-amplified and sequenced. Analysis was undertaken using the mothur package. Volatile organic compounds (VOC) were also measured. Results Ninety-one individuals were in the primary analysis (37 CD, 30 bioresource controls, and 24 household controls). Comparing CD with nonIBD controls, there were reductions in bacterial diversity, Ruminococcaceae, Rikenellaceae, and Christensenellaceae and an increase in Enterobacteriaceae. No significant differences could be identified in microbiota by NOD2 genotype, but fecal butanoic acid was higher in Crohn's patients carrying NOD2 mutations. Conclusions In this well-controlled study of NOD2 genotype and fecal microbiota, we identified no significant genotype-microbiota associations. This suggests that the changes associated with NOD2 genotype might only be seen at the mucosal level, or that environmental factors and prior inflammation are the predominant determinant of the observed dysbiosis in gut microbiota. |
| Starting Page | 583 |
| Ending Page | 592 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://aura.abdn.ac.uk/bitstream/handle/2164/10060/The_Impact_of_NOD2_Variants_on_Fecal_Microbiota_in_Crohn_s_Disease_and_Controls_Without_Gastrointestinal_Disease.pdf?isAllowed=y&sequence=1 |
| PubMed reference number | 29462388v1 |
| Alternate Webpage(s) | https://doi.org/10.1093/ibd/izx061 |
| DOI | 10.1093/ibd/izx061 |
| Journal | Inflammatory bowel diseases |
| Volume Number | 24 |
| Issue Number | 3 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bacteria Butyric Acid CDKN2A gene Christensenellaceae Crohn Disease Dysbiosis Fecal Microbiota Transplantation Fecal occult blood test Gastrointestinal Diseases Immunity, Innate Intestinal Microbiome Irritable Bowel Syndrome Mental association Microbiota (plant) Mucous Membrane Mutation NOD2 gene Patients Polymerase Chain Reaction Volatile Organic Compounds |
| Content Type | Text |
| Resource Type | Article |
