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The crystal structure of D-lactate dehydrogenase, a peripheral membrane respiratory enzyme.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dym, Orly Pratt, Erika Ho, Chien Eisenberg, David S. |
| Copyright Year | 2000 |
| Abstract | d-Lactate dehydrogenase (d-LDH) of Escherichia coli is a peripheral membrane respiratory enzyme involved in electron transfer, located on the cytoplasmic side of the inner membrane. d-LDH catalyzes the oxidation of d-lactate to pyruvate, which is coupled to transmembrane transport of amino acids and sugars. Here we describe the crystal structure at 1.9 A resolution of the three domains of d-LDH: the flavin adenine dinucleotide (FAD)-binding domain, the cap domain, and the membrane-binding domain. The FAD-binding domain contains the site of d-lactate reduction by a noncovalently bound FAD cofactor and has an overall fold similar to other members of a recently discovered FAD-containing family of proteins. This structural similarity extends to the cap domain as well. The most prominent difference between d-LDH and the other members of the FAD-containing family is the membrane-binding domain, which is either absent in some of these proteins or differs significantly. The d-LDH membrane-binding domain presents an electropositive surface with six Arg and five Lys residues, which presumably interacts with the negatively charged phospholipid head groups of the membrane. Thus, d-LDH appears to bind the membrane through electrostatic rather than hydrophobic forces. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://people.mbi.ucla.edu/david/Reprints/Dym,%20The%20Crystal%20Structure%20of%20D-Lactate%20Dehydrogenase,%20PNAS%202000.pdf |
| PubMed reference number | 10944213v1 |
| Volume Number | 97 |
| Issue Number | 17 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 4,6-dinitro-o-cresol Adenine Amino Acids Charge (electrical) Dinucleoside Phosphates Electron Transport Flavin-Adenine Dinucleotide L-Lactate dehydrogenase (cytochrome) Lactate Dehydrogenase Lactic acid Lysine Phospholipids Pyruvates Respiratory Insufficiency Sugars Tissue membrane Transmembrane Transport chemical cofactor oxidation |
| Content Type | Text |
| Resource Type | Article |
