NDLI: The structure of the COPI coat determined within the cell

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The structure of the COPI coat determined within the cell

Content Provider	Semantic Scholar
Author	Bykov, Yury S. Schaffer, Miroslava Dodonova, Svetlana O. Albert, Sahradha Plitzko, Juergen M. Baumeister, Wolfgang Engel, Benjamin D. Briggs, John A. G.
Copyright Year	2017
Abstract	COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with in vitro reconstitution systems using purified components. Previously we have determined a complete structural model of the in vitro reconstituted COPI coat (Dodonova et al., 2017). Here, we applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells. The native algal structure resembles the in vitro mammalian structure, but additionally reveals cargo bound beneath β'-COP. We find that all coat components disassemble simultaneously and relatively rapidly after budding. Structural analysis in situ, maintaining Golgi topology, shows that vesicles change their size, membrane thickness, and cargo content as they progress from cis to trans, but the structure of the coat machinery remains constant.
Starting Page	6916
Ending Page	6921
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://elifesciences.org/download/aHR0cHM6Ly9jZG4uZWxpZmVzY2llbmNlcy5vcmcvYXJ0aWNsZXMvMzI0OTMvZWxpZmUtMzI0OTMtdjIucGRm/elife-32493-v2.pdf?_hash=IN2sb7Hnjro3yxJnh1jYgb3S1QmTMF0UWptmAtCBdOs%3D
PubMed reference number	29148969
Alternate Webpage(s)	https://doi.org/10.7554/eLife.32493
DOI	10.7554/elife.32493
Journal	eLife
Volume Number	6
Language	English
Access Restriction	Open
Subject Keyword	Anatomy, Regional COPI-Coated Vesicles Chlamydomonas reinhardtii Coat Protein Complex I Coated vesicle Disassembly (action) ER to Golgi vesicle-mediated transport Endoplasmic Reticulum Golgi Apparatus Ions Mammals Membrane Proteins Tissue membrane Tomography, Emission-Computed Vesicle (morphologic abnormality) tomography
Content Type	Text
Resource Type	Article

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