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Rer1p, a Retrieval Receptor for Endoplasmic Reticulum Membrane Proteins, Is Dynamically Localized to the Golgi Apparatus by Coatomer
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sato, Ken Sato, Miyuki Nakano, Akihiko |
| Copyright Year | 2001 |
| Abstract | Rer1p, a yeast Golgi membrane protein, is required for the retrieval of a set of endoplasmic reticulum (ER) membrane proteins. We present the first evidence that Rer1p directly interacts with the transmembrane domain (TMD) of Sec12p which contains a retrieval signal. A green fluorescent protein (GFP) fusion of Rer1p rapidly cycles between the Golgi and the ER. Either a lesion of coatomer or deletion of the COOH-terminal tail of Rer1p causes its mislocalization to the vacuole. The COOH-terminal Rer1p tail interacts in vitro with a coatomer complex containing alpha and gamma subunits. These findings not only give the proof that Rer1p is a novel type of retrieval receptor recognizing the TMD in the Golgi but also indicate that coatomer actively regulates the function and localization of Rer1p. |
| Starting Page | 935 |
| Ending Page | 944 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 11238450 |
| Volume Number | 152 |
| Journal | The Journal of cell biology |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/ae/36/0011122.PMC2198819.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | COPI-Coated Vesicles Deletion Mutation Endoplasmic Reticulum Golgi Apparatus Green Fluorescent Proteins Membrane Proteins Transmembrane Domain Vacuole positive regulation of ER to Golgi vesicle-mediated transport receptor viral capsid secondary envelopment |
| Content Type | Text |
| Resource Type | Article |
