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Rapid Determination of Lipid Accumulation Under Sulfur Starvation in Chlamydomonas reinhardtii Microalga Using Fourier Transform Infrared ( FTIR ) spectroscopy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Morowvat, Mohammad Hossein Ghasemi, Younes |
| Copyright Year | 2016 |
| Abstract | Chlamydomonas reinhardtii, the most commonly studied model organisms in microalgal kingdom, is known to be a rich lipid producer. Here, a naturally isolated Chlamydomonas reinhardtii strain was cultivated phototrophically at Erlenmeyer scale in BG11 culture medium. After reaching to stationary growth phase, a sulfur limited regime was applied. The lipid content of C. reinhardtii microalga, was determined using Fourier transform infrared micro-spectroscopy (FTIR) method in Erlenmeyer flasks in normal and sulfur starved conditions. After sulfur limitation the microalgal cells were significantly decreased (1.582 g L) in comparison with grown cells in sulfur rich medium (4.484 g L). The results indicate an elevated lipid: amide I in sulfur rich medium (1.66-fold) compared with sulfur rich medium. Gravimetric analysis of the total lipid contents, as a conventional method, confirmed that the lipid accumulation (36.98%) was due to elevated lipid biosynthesis. The results open a new way to direct quantification of total lipids in more microalgal strains using FTIR as a reliable and robust method. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://impactfactor.org/PDF/IJPCR/8/IJPCR,Vol8,Issue9,Article17.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
