NDLI: CrABCA2 Facilitates Triacylglycerol Accumulation in <i>Chlamydomonas reinhardtii</i> under Nitrogen Starvation.

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CrABCA2 Facilitates Triacylglycerol Accumulation in Chlamydomonas reinhardtii under Nitrogen Starvation.

Content Provider	Europe PMC
Author	Jang, Sunghoon Kong, Fantao Lee, Jihyeon Choi, Bae Young Wang, Pengfei Gao, Peng Yamano, Takashi Fukuzawa, Hideya Kang, Byung-Ho Lee, Youngsook
Copyright Year	2020
Abstract	The microalga Chlamydomonas reinhardtii accumulates triacylglycerols (TAGs) in lipid droplets under stress conditions, such as nitrogen starvation. TAG biosynthesis occurs mainly at the endoplasmic reticulum (ER) and requires fatty acid (FA) substrates supplied from chloroplasts. How FAs are transferred from chloroplast to ER in microalgae was unknown. We previously reported that an Arabidopsis thaliana ATP-binding cassette (ABC) transporter, AtABCA9, facilitates FA transport at the ER during seed development. Here we identified a gene homologous to AtABCA9 in the C. reinhardtii genome, which we named CrABCA2. Under nitrogen deprivation conditions, CrABCA2 expression was upregulated, and the CrABCA2 protein level also increased. CrABCA2 knockdown lines accumulated less TAGs and CrABCA2 overexpression lines accumulated more TAGs than their untransformed parental lines. Transmission electron microscopy showed that CrABCA2 was localized in swollen ER. These results suggest that CrABCA2 transports substrates for TAG biosynthesis to the ER during nitrogen starvation. Our study provides a potential tool for increasing lipid production in microalgae.
ISSN	10168478
Journal	Molecules and Cells
Volume Number	43
PubMed Central reference number	PMC6999713
Issue Number	1
PubMed reference number	31910336
e-ISSN	02191032
DOI	10.14348/molcells.2019.0262
Language	English
Publisher	Korean Society for Molecular and Cellular Biology
Publisher Date	2020-01-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/. © The Korean Society for Molecular and Cellular Biology. All rights reserved.
Subject Keyword	ABC transporter Chlamydomonas reinhardtii endoplasmic reticulum triacylglycerol accumulation
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Molecular Biology

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