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HPLC Determination of α-keto Acids from Human Serum Using 2,3-Diamino-2,3-dimethylbutane as Derivatizing Reagent
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mahar, Khalida Parveen Khuhawar, Muhammad Yar Shar, Gulam Qadir Azmat, Rafia Baig, Jameel Ahmed |
| Copyright Year | 2013 |
| Abstract | Seven α-keto acids, pyruvic acid (PYR), 2-oxobutyric acid (KB), 3-methyl-2-oxobutyric acid (MKBA), 3-methyl-2-oxovaleric acid (K3MVA), 4-methyl-2-oxovaleric acid (K4MVA), 2- oxoglutaric acid (KG) and Phenyl pyruvic acid (PPY) as derivatives of 2,3-diamino-2,3- dimethybutane (DDB) were separated by HPLC column Zorbax C-18 (4.6x150 mm-id). The compounds were eluted with methanol-water-acetonitrile (40:58:2 V/V/V) with flow rate 1 ml/min. UV detection was carried out by photodiode array at 255 nm. Linear calibration plots were obtained with 0.1 to 60 µg/ml with limits of detection (LoD) within 0.04-0.4 µg/ml. The method was applied for the analysis of α-keto acids from serum of diabetic patients with blood glucose level 430-458 mg/dl and healthy volunteers. The amounts of α-keto acids observed 3.24- 9.71 µg/ml with RSD 1.1-1.9% in diabetic patients were higher than healthy volunteer's 0.11-1.3 µg/ml with RSD 0.9-2.6%. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ceacsu.edu.pk/PDF%20file/Volume%2014%20No%202/8-15-PJAECDDP-25082013-21-1.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
