NDLI: High Performance Liquid Chromatographic Determination Of α-keto Acids From A Pharmaceutical Preparation (Tablet) Using 4-Nitro-1, 2-Phenylene Diamine As A Derivatizing Reagent

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High Performance Liquid Chromatographic Determination Of α-keto Acids From A Pharmaceutical Preparation (Tablet) Using 4-Nitro-1, 2-Phenylene Diamine As A Derivatizing Reagent

Content Provider	Semantic Scholar
Author	Mahar, Khalida Parveen Khuhawar, Muhammad Yar Abbasi, Kulsoom Azmat, Rafia Mastoi, Ghulam Murtaza Channer, Adbul Hmeed
Copyright Year	2012
Abstract	A rapid, sensitive and selective HPLC separation with photodiode array detector was developed for the determination of the αketo acids from pharmaceutical tablet. α-keto acids (1) 3-methyl 2-oxobutyric acid (MKBA), (2) 3-methyl 2-oxovaleric acid (K3MVA), (3) 4-methyl-2-oxovaleric acid (K4MVA) and (4) Phenyl pyruvic acid (PPY) as derivatives with 4-dinitro 1,2phenylenediamine (NPD) eluted and separated from HPLC column Zorbax C–18 and the UV detection was carried out by photodiode array at 255 nm at pH 3. The isocratic elution was with methanol-water-acetonitrile (42:56:2V/V/V) with flow rate 0.9 ml/min. The standard curves were linear from 0.45-100 μg/ml and detection limits were within 0.1-0.266 μg/ml of each acid. The elution and separation of four keto acids was rapid within 10 min.The method was repeatable with relative standard deviation (RSD) within 0.1-2.9% for each of the α-keto acids. The method was applied for determination of α-keto acids from a pharmaceutical preparation. The result was further confirmed by standard addition technique.
Starting Page	29
Ending Page	33
Page Count	5
File Format	PDF HTM / HTML
DOI	10.15228/2012.v02.i01.p05
Volume Number	2
Alternate Webpage(s)	https://core.ac.uk/download/pdf/25536215.pdf
Alternate Webpage(s)	https://doi.org/10.15228/2012.v02.i01.p05
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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