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Real-time PCR detection of Moritella viscosa, the likely causal agent of winter-ulcer in Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Grove, Søren Reitan, Liv Jorun Lunder, Tjaša Colquhoun, David |
| Copyright Year | 2008 |
| Abstract | We report the development of a real-time PCR protocol for specific detection of Moritella viscosa. This bacterium is considered to be the main aetiological agent in development of winter-ulcer, a disease severely affecting salmonid aquaculture in Norway. From a newly elaborated draft version of the genome of M. viscosa, the tonB gene sequence was selected as a suitable basis for the development of the real-time PCR assay. The real-time PCR demonstrated the presence of M. viscosa DNA sequences in 88.1% of samples collected from 35 outbreaks of winter-ulcer in Norwegian fish farms. In contrast, standard culturing on blood agar identified M. viscosa in only 39.7% of fish. While the culturing method revealed a similar prevalence (26 to 27%) of M. viscosa in kidney and ulcer samples, substantially more ulcer (81.5%) than kidney (49.7%) samples were shown positive by real-time PCR. |
| File Format | PDF HTM / HTML |
| DOI | 10.3354/dao01972 |
| PubMed reference number | 19149373 |
| Journal | Medline |
| Volume Number | 82 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://www.int-res.com/articles/dao2008/82/d082p105.pdf |
| Alternate Webpage(s) | https://doi.org/10.3354/dao01972 |
| Journal | Diseases of aquatic organisms |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
