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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Grove, S. Reitan, L. J. Lunder, T. Colquhoun, D. |
| Description | Country affiliation: Norway Author Affiliation: Grove S ( National Veterinary Institute, PO Box 8156 Dep, 0033 Oslo, Norway. soren.grove@vetinst.no) |
| Abstract | We report the development of a real-time PCR protocol for specific detection of Moritella viscosa. This bacterium is considered to be the main aetiological agent in development of winter-ulcer, a disease severely affecting salmonid aquaculture in Norway. From a newly elaborated draft version of the genome of M. viscosa, the tonB gene sequence was selected as a suitable basis for the development of the real-time PCR assay. The real-time PCR demonstrated the presence of M. viscosa DNA sequences in 88.1% of samples collected from 35 outbreaks of winter-ulcer in Norwegian fish farms. In contrast, standard culturing on blood agar identified M. viscosa in only 39.7% of fish. While the culturing method revealed a similar prevalence (26 to 27%) of M. viscosa in kidney and ulcer samples, substantially more ulcer (81.5%) than kidney (49.7%) samples were shown positive by real-time PCR. |
| File Format | HTM / HTML |
| ISSN | 01775103 |
| e-ISSN | 16161580 |
| Journal | Diseases of Aquatic Organisms |
| Issue Number | 2 |
| Volume Number | 82 |
| Language | English |
| Publisher | Federal Republic of Germany : Inter-Research |
| Publisher Date | 2008-11-20 |
| Publisher Place | Germany |
| Access Restriction | Open |
| Subject Keyword | Discipline Veterinary Medicine Discipline Biology Fish Diseases Microbiology Gram-negative Bacterial Infections Veterinary Moritella Isolation & Purification Oncorhynchus Mykiss Polymerase Chain Reaction Salmo Salar Animals Bacteriological Techniques Sensitivity And Specificity Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Aquatic Science Ecology, Evolution, Behavior and Systematics |
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