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Mechanism of cyclic AMP facilitation of stimulation-evoked catecholamine release in adrenal chromaffin cells—II. Inhibition of Na+,K+-ATPase by cyclic AMP
| Content Provider | Semantic Scholar |
|---|---|
| Author | Morita, Katsuya Dohi, Toshihiro Minami, Naoki Kitayama, Shigeo Tsujimoto, Akira |
| Copyright Year | 1991 |
| Abstract | Abstract The ability of cyclic AMP to inhibit Na + ,K + -ATPase activity in adrenal medulla was examined to elucidate mechanisms of cyclic AMP stimulatory modulations in catecholamine (CA) secretion from adrenal gland. Cyclic AMP, 10 −7 –10 −4 M, was demonstrated to inhibit Na + ,K + -ATPase activity of microsomes from bovine adrenal medulla. 8-Br cyclic AMP and forskolin reduced [γ- 32 P]ATP hydrolysis by digitonin-permeabilized chromaffin cells and this reduction of ATP hydrolysis was due to an inhibition of Na + ,K + -ATPase. Catalytic subunit of cyclic AMP-dependent protein kinase also reduced [γ- 32 P]ATP hydrolysis and the inhibitor of the protein kinase blocked the catalytic subunit-inducted reduction of ATP hydrolysis. 8-Br cyclic AMP and forskolin inhibited 86 Rb uptake into cultured chromaffin cells. From these results, the ability of cyclic AMP to inhibit adrenomedullary Na + ,K + -ATPase was shown in crude membrane fraction, in leaky cell preparation and also in intact cells. |
| Starting Page | 81 |
| Ending Page | 85 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0197-0186(91)90122-T |
| Volume Number | 19 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/019701869190122T |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/019701869190122T?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0197-0186%2891%2990122-T |
| Journal | Neurochemistry International |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
