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Stimulation-Evoked Ca2+Fluxes in Cultured Bovine Adrenal Chromaffin Cells Are Enhanced by Forskolin
| Content Provider | Scilit |
|---|---|
| Author | Morita, Katsuya Dohi, Toshihiro Kitayama, Shigeo Kyama, Yutaka Tsujimoto, Akira |
| Copyright Year | 1987 |
| Description | Journal: Journal of Neurochemistry Forskolin, 1 microM, increased acetylcholine (ACh)-stimulated 45Ca uptake by chromaffin cells. The stimulatory effects of forskolin decreased with increasing concentration of ACh. The attenuation of the effect of forskolin on 45Ca uptake as a function of ACh concentration correlated well with changes in the forskolin effect on ACh-evoked catecholamine (CA) release. Forskolin increased excess KCl- and veratrine-evoked CA release and 45Ca uptake. Forskolin by itself stimulated 45Ca efflux and enhanced ACh-, excess KCl-, and veratrine-stimulated 45Ca efflux. High doses of forskolin inhibited both ACh-evoked 45Ca uptake and CA release. The inhibitory action of forskolin was specific to receptor-mediated response because excess KCl- and veratrine-stimulated 45Ca uptake and CA release were not inhibited. Forskolin, 0.3-30 microM, dose-dependently increased caffeine-stimulated CA release and 45Ca efflux in the absence of Ca2+ in the medium, and the effects were mimicked by dibutyryl cyclic AMP. These results suggest that cyclic AMP increases stimulation-induced CA release by enhancing calcium uptake across the plasma membrane and/or altering calcium flux in an intracellular calcium store. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1987.tb13155.x/pdf |
| Ending Page | 252 |
| Page Count | 5 |
| Starting Page | 248 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1987.tb13155.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 1 |
| Volume Number | 48 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1987-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Endocrinology and Metabolism Cyclic Amp Catecholamine Release Calcium Uptake Calcium Efflux Adrenal Chromaffin Cells. Morita K. Et Al |
| Content Type | Text |
| Resource Type | Article |
