NDLI: Architecture of the budding yeast kinetochore reveals a conserved molecular core

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Architecture of the budding yeast kinetochore reveals a conserved molecular core

Content Provider	Semantic Scholar
Author	Westermann, Stefan Cheeseman, Iain M. Anderson, Scott T. Yates, John R. Drubin, David G. Barnes, Georjana
Copyright Year	2003
Abstract	How kinetochore proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores from distantly related organisms, are key unanswered questions. Here, we used affinity chromatography and mass spectrometry to generate a map of kinetochore protein interactions. The budding yeast CENP-C homologue Mif2p specifically copurified with histones H2A, H2B, and H4, and with the histone H3-like CENP-A homologue Cse4p, strongly suggesting that Cse4p replaces histone H3 in a specialized centromeric nucleosome. A novel four-protein Mtw1 complex, the Nnf1p subunit of which has homology to the vertebrate kinetochore protein CENP-H, also copurified with Mif2p and a variety of central kinetochore proteins. We show that Mif2 is a critical in vivo target of the Aurora kinase Ipl1p. Chromatin immunoprecipitation studies demonstrated the biological relevance of these associations. We propose that a molecular core consisting of CENP-A, -C, -H, and Ndc80/HEC has been conserved from yeast to humans to link centromeres to spindle microtubules.
Starting Page	215
Ending Page	222
Page Count	8
File Format	PDF HTM / HTML
PubMed reference number	14581449
Volume Number	163
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/163/2/215.full.pdf?with-ds=yes
Language	English
Access Restriction	Open
Subject Keyword	CENPC gene Centromere Chromatography, Affinity Chromosomes Histone H2a Histone H2b Histones Homologous Gene Kinetochores Mental association Saccharomycetales Spectrometry nucleosome location
Content Type	Text
Resource Type	Article

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