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Mediator-free phenol sensor based on titania sol-gel encapsulation matrix for immobilization of tyrosinase by a vapor deposition method.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yu, Jiuhong Liu, Songqin Ju, Huangxian |
| Copyright Year | 2003 |
| Abstract | A novel amperometric phenol sensor was constructed by immobilizing tyrosinase in a titania sol-gel matrix. The tyrosinase entrapped sol-gel film was obtained with a vapor deposition method, which simplified the traditional sol-gel process and avoided the shrinkage and cracking of conventional sol-gel-derived glasses. This matrix provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenol could be oxidized by dissolving oxygen in presence of immobilized tyrosinase to form a detectable product, which was determined at -150 mV without any mediator. The phenol sensor exhibited a fast response (less than 5 s) and sensitivity as high as 103 microA/mM, which resulted from the porous structure and high enzyme loading of the sol-gel matrix. The linear range for phenol determination was from 1.2x10(-7) to 2.6x10(-4) M with a detection limit of 1.0x10(-7) M. The apparent Michaelis-Menten constant of the encapsulated tyrosinase was calculated to be (0.29+/-0.02) mM. The stability of the biosensor was also evaluated. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0956-5663(03)00227-6 |
| PubMed reference number | 14623476 |
| Journal | Medline |
| Volume Number | 19 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://sklac.nju.edu.cn/hxju/lunwenlunzhu/paper2001-2003/120%20BB%20Yu%20JH.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S0956-5663%2803%2900227-6 |
| Journal | Biosensors & bioelectronics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
