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TEM-121, a novel complex mutant of TEM-type beta-lactamase from Enterobacter aerogenes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Poirel, Laurent Mammeri, Hedi Nordmann, Patrice L. |
| Copyright Year | 2004 |
| Abstract | Enterobacter aerogenes clinical isolate LOR was resistant to penicillins and ceftazidime but susceptible to cefuroxime, cephalothin, cefoxitin, cefotaxime, ceftriaxone, and cefepime. PCR and cloning experiments from this strain identified a novel TEM-type beta-lactamase (TEM-121) differing by five amino acid substitutions from beta-lactamase TEM-2 (Glu104Lys, Arg164Ser, Ala237Thr, Glu240Lys, and Arg244Ser) and by only one amino acid change from the extended-spectrum beta-lactamase (ESBL) TEM-24 (Arg244Ser), with the last substitution also being identified in the inhibitor-resistant beta-lactamase IRT-2. Kinetic parameters indicated that TEM-121 hydrolyzed ceftazidime and aztreonam (like TEM-24) and was inhibited weakly by clavulanic acid and strongly by tazobactam. Thus, TEM-121 is a novel complex mutant TEM beta-lactamase (CMT-4) combining the kinetic properties of an ESBL and an inhibitor-resistant TEM enzyme. |
| Starting Page | 3 |
| Ending Page | 11 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://aac.asm.org/content/48/12/4528.full.pdf |
| PubMed reference number | 15561821v1 |
| Volume Number | 48 |
| Issue Number | 12 |
| Journal | Antimicrobial agents and chemotherapy |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acid Substitution Amino Acids Aztreonam Cefotaxime Cefoxitin Ceftazidime Ceftriaxone Cefuroxime Cephalothin Clavulanic Acid Enterobacter aerogenes Kinetics LOR gene Penicillins beta-Lactamase cefepime tazobactam |
| Content Type | Text |
| Resource Type | Article |
