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Microfluidic bead encapsulation above 20 kHz with triggered drop formation† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8lc00514a
| Content Provider | Semantic Scholar |
|---|---|
| Author | Clark, Iain C. Abate, Adam R. |
| Copyright Year | 2018 |
| Abstract | Microsphere beads are functionalized with oligonucleotides, antibodies, and other moieties to enable specific detection of analytes. Droplet microfluidics leverages this for single-molecule or -cell analysis by pairing beads and targets in water-in-oil droplets. Pairing is achieved with devices operating in the dripping regime, limiting throughput. Here, we describe a pairing method that uses beads to trigger the breakup of a jet into monodispersed droplets. We use the method to pair 105 Human T cells with polyacrylamide beads ten times faster than methods operating in the dripping regime. Our method improves the throughput of bead-based droplet workflows, enabling analysis of large populations and the detection of rare events. |
| Starting Page | 3598 |
| Ending Page | 3605 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/c8lc00514a |
| PubMed reference number | 30362490 |
| Journal | Medline |
| Volume Number | 18 |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/articlepdf/2018/lc/c8lc00514a?page=search |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/articlepdf/2018/lc/c8lc00514a |
| Alternate Webpage(s) | https://doi.org/10.1039/c8lc00514a |
| Journal | Lab on a chip |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
