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Rearing of Exorista larvarum (Diptera Tachinidae): simplification of the in vitro technique
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dindo, Maria Luisa Marchetti, Elisa Galvagni, Giovanna Baronio, Piero |
| Copyright Year | 2003 |
| Abstract | The tachinid Exorista larvarum (L.) was developed in vitro from egg to adult using absorbent cotton in replacement of more expensive agar as a physical support of the artificial medium. Puparial yields and adult emergence did not differ significantly between agar-containing and cotton absorbed diets. The heaviest puparia (mg 66-69 for males and 59-60 for females) were obtained when 15 mg cotton were used to absorb 0.4 ml diet. With this ratio of absorbent mass to volume of absorbed liquid, puparial yields reached as high as 61% and over 92% of puparia emerged as adults. Subsequently a comparison was made of in vitro rearing procedure conducted in or out of a laminar flow hood with a cotton absorbed diet devoid of antifungal agents. When the procedure was conducted out of a laminar flow hood no significant increase of mould contamination or decrease of adult yields occurred. The weights of male puparia were significantly lighter than the weights of puparia reared in a laminar flow hood. Both male and female puparia obtained out of a laminar flow hood however weighed more than those previously cultured in vivo from monoparasitized G. mellonella larvae. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bulletinofinsectology.org/pdfarticles/vol56-2003-253-257dindo.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
