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In vitro rearing of the tachinid parasitoid Exorista larvarum with exclusion of the host insect for more than one generation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Marchetti, Elisa Baronio, Piero Dindo, Maria Luisa |
| Copyright Year | 2008 |
| Abstract | We tested the possibility to eliminate the host insect from the line of production of Exorista larvarum (L.), a larval parasitoid of Lepidoptera, for more than one generation. Galleria mellonella (L.) larvae or a plastic sheet were provided as oviposition substrates either to parental or 1 st generation females. The eggs/female laid in 45 min were dramatically fewer on the plastic sheet compared to host larvae and decreased from the parental to the 1 st generation. Both the 1 st and 2 nd generation eggs (respectively laid by parental and 1 st generation females) were removed from the oviposition substrates (host larvae or plastic sheet) and placed on a skimmed milk-based artificial medium. The percentages of hatched eggs were not significantly affected by the oviposition substrate, but the generation effect was significant and fewer eggs hatched in the 2 nd than in the 1 st generation. The percentages of puparia and adults were not significantly influenced either by the oviposition substrate or generation. All the 2 nd generation adults obtained from eggs laid on the plastic sheet died 1-2 days after emergence, before the females oviposited, whereas the 2 nd generation adults obtained from eggs removed from the host integument survived. After mating, the females laid eggs on G. mellonella larvae. The results confirmed that, for one generation, the out-of-host eggs are competitive with those removed from G. mellonella larvae to rear E. larvarum on an artificial medium. The quality of the in vitro-cultured tachinids however decreases over generations, especially if the host is completely excluded from their line of production, i.e. not even used for collecting eggs. Moreover, several factors need to be investigated for E. larvarum to be stimulated to lay more eggs on artificial substrates. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bulletinofinsectology.org/pdfarticles/vol61-2008-333-336marchetti.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
