NDLI: High-resolution computed tomography with a compact soft x-ray microscope.

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High-resolution computed tomography with a compact soft x-ray microscope.

Content Provider	Semantic Scholar
Author	Bertilson, Michael C. Hofsten, Olov Von Vogt, Ulrich F. Holmberg, Anders Hertz, Hans M.
Copyright Year	2009
Abstract	Computed tomography based on high-resolution soft x-ray microscopy utilizes the natural contrast for biological specimens provided by the water window (lambda = 2.4 - 4.4 nm) and the high resolving power of zone plate objectives. It is capable of revealing the 3D structure of biological specimens at sub-visible-microscopic resolution. To date, the technique has only been available at synchrotron-based microscopes, which limits the researchers access. In the present paper we demonstrate high-resolution soft x-ray tomography with a laboratory zone-plate-based soft x-ray microscope. The specimen, a diatom mounted on a glass capillary, was reconstructed from a tilt series of 53 images covering 180 degrees using a filtered back projection algorithm. The resolution of the tomogram was estimated to a half period of 140 nm using a differential-phase-residual method. Cryo-fixation, increased source brightness and extended-depth-of-focus objectives are important for pushing the resolution of compact systems for biological samples.
Starting Page	19
Ending Page	38
Page Count	20
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://xrm.phys.northwestern.edu/research/pdf_papers/2009/bertilson_oe_2009.pdf
PubMed reference number	19550505v1
Volume Number	17
Issue Number	13
Journal	Optics express
Language	English
Access Restriction	Open
Subject Keyword	Blood capillaries CDISC SEND Biospecimens Terminology Diagnostic radiologic examination Diatoms Diode Device Component Kilohertz Large Microscope Device Component Neodymium-Doped Yttrium Aluminum Garnet Lasers OSA protein, Drosophila Optics Photons Plain x-ray Projection Defense Mechanism Projections and Predictions Radiation Damage Revision procedure Roentgen Rays Scientific Publication Sodium Specimen Stage Is Synchrotrons Tomography, Emission-Computed Tomography, X-Ray X-Ray Computed Tomography algorithm brightness julia pump (device)
Content Type	Text
Resource Type	Article

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