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Electron microscope tomography: transcription in three dimensions.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Olins, Donald E. Olins, Ada L. Levy, Henri A. Durfee, References Margle, S. M. Tinnel, E. P. Dover, Sharon |
| Copyright Year | 1983 |
| Abstract | Three-dimensional reconstruction of an asymmetric biological ultrastructure has been achieved by tomographic analysis of electron micrographs of sections tilted on a goniometer specimen stage. Aligned micrographs could be displayed as red-green three-dimensional movies. The techniques have been applied to portions of in situ transcription units of a Balbiani ring in the polytene chromosomes of the midge Chironomus tentans. Current data suggest a DNA compaction of about 8 to 1 in a transcription unit. Nascent ribonucleoprotein granules display an imperfect sixfold helical arrangement around the chromatin axis. |
| Starting Page | 498 |
| Ending Page | 500 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://science.sciencemag.org/content/sci/220/4596.toc.pdf |
| PubMed reference number | 6836293v1 |
| Volume Number | 220 |
| Issue Number | 4596 |
| Journal | Science |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alignment Axis vertebra CDISC SEND Biospecimens Terminology Chironomus thummi Ab.IgE:ACnc:Pt:Ser:Qn Dimensions Granule Microscope Device Component Movies P1 Bacteriophage Artificial Chromosomes Ribonucleoproteins Scanning Electron Microscopy X-Ray Computed Tomography |
| Content Type | Text |
| Resource Type | Article |
