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Characterization of the apoptosis suppressor protein P49 from the Spodoptera littoralis nucleopolyhedrovirus.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pei, Zifei Reske, Galit Huang, Q. Hammock, Bruce D. Qi, Yipeng Chejanovsky, Nor |
| Copyright Year | 2002 |
| Abstract | Two antiapoptotic types of genes, iap and p35, were found in baculoviruses. P35 is a 35-kDa protein that can suppress apoptosis induced by virus infection or by diverse stimuli in vertebrates or invertebrates. iap homologues were identified in insects and mammals. Recently, we have identified sl-p49, a novel apoptosis suppressor gene and the first homologue of p35, in the genome of the Spodoptera littoralis nucleopolyhedrovirus. Here we show that sl-p49 encodes a 49-kDa protein, confirmed its primary structure that displays 48.8% identity to P35, and performed computer-assisted modeling of P49 based on the structure of P35. We demonstrated that P49 is able to inhibit insect and human effector caspases, which requires P49 cleavage at Asp(94). Finally we identified domains important for P49's antiapoptotic function that include a reactive site loop (RSL) protruding from a beta-barrel domain. RSL begins at an amphipathic alpha1 helix, traverses the beta-sheet central region, exposing Asp(94) at the apex, and rejoins the beta-barrel. Our model predicted seven alpha-helical motifs, three of them unique to P49. alpha-Helical motifs alpha(1), alpha(2), and alpha(4') were required for P49 function. The high structural homology between P49 and P35 suggests that these molecules bear a scaffold common to baculovirus "apoptotic suppressor" proteins. P49 may serve as a novel tool to analyze the contribution of different components of the caspase chain in the apoptotic response in organisms not related phylogenetically. |
| Starting Page | 1 |
| Ending Page | 2 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://ucanr.edu/sites/hammocklab/files/131033.pdf |
| PubMed reference number | 12324475v1 |
| Volume Number | 277 |
| Issue Number | 50 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | APEX1 protein, human Antiapoptotic Agent Apoptosis Baculovirus Binding Sites Caspases, Effector Genes, Suppressor Homologous Gene Invertebrates Mammals Nucleopolyhedrovirus Primase Polypeptide 1 Spodoptera Vertebrates caspase type I interferon receptor |
| Content Type | Text |
| Resource Type | Article |
