NDLI: Real-time reverse transcriptase-polymerase chain reaction quantification of West Nile virus transmitted by Culex pipiens quinquefasciatus.

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Real-time reverse transcriptase-polymerase chain reaction quantification of West Nile virus transmitted by Culex pipiens quinquefasciatus.

Content Provider	Semantic Scholar
Author	Vanlandingham, Dana L. Schneider, Bradley S. Klingler, Kimberly Fair, Joseph N. Beasley, David Huang, Jing Hamilton, Patricia Higgs, Stephen T.
Copyright Year	2004
Abstract	Transmission experiments are a critical component of vector competence studies. In this study, a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was used to enumerate the amount of West Nile virus (WNV) secreted in mosquito saliva following oral infection. Culex pipiens quinquefasciatus were allowed to feed on WNV-infected blood, and saliva was collected on days 14 and 21 post-infection (pi). The amount of virus at these two time points varied significantly, with mean equivalent plaque-forming units (pfu) of approximately 30,500 on day 14 pi and 5,800 on day 21 pi. Individual mosquitoes secreted up to 2 x 10(5) pfu of virus. Titer of whole mosquitoes and immunofluorescence assay of salivary glands from mosquitoes collected at these two time points were also used for supplemental comparison. This report describes the first use of a real-time RT-PCR to quantify the amount of WNV in mosquito saliva.
Starting Page	1613
Ending Page	1615
Page Count	3
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://intl.ajtmh.org/content/71/1/120.full.pdf
Alternate Webpage(s)	http://www.ajtmh.org/content/71/1/120.full.pdf
PubMed reference number	15238700v1
Volume Number	71
Issue Number	1
Journal	The American journal of tropical medicine and hygiene
Language	English
Access Restriction	Open
Subject Keyword	Cell secretion Culex (Genus) Culicidae Dental Plaque Fluorescent Antibody Technique Gland Murine sarcoma viruses Quantitation RNA-Directed DNA Polymerase Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Salivary Glands West Nile virus disease transmission oral infection saliva
Content Type	Text
Resource Type	Article

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