NDLI: West Nile virus infection alters midgut gene expression in Culex pipiens quinquefasciatus Say (Diptera: Culicidae).

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West Nile virus infection alters midgut gene expression in Culex pipiens quinquefasciatus Say (Diptera: Culicidae).

Content Provider	Semantic Scholar
Author	Smartt, Chelsea T. Richards, Stephanie L. Anderson, Sheri L. Erickson, Jennifer Sien
Copyright Year	2009
Abstract	Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding on infected blood. Of these, 21 cDNAs showed an increase in expression, and 5 showed a decrease in expression as a result of WNV presence in the blood meal. GenBank database searches showed that one clone with increased expression, CQ G12A2, shares 94% identity with a leucine-rich repeat-containing protein from Cx. p. quinquefasciatus and 32% identity to Toll-like receptors from Aedes aegypti. We present the first cDNA clone isolated from female Cx. p. quinquefasciatus midgut tissue whose expression changes on exposure to WNV. This cDNA represents a mosquito gene that is an excellent candidate for interacting with WNV in Cx. p. quinquefasciatus and may play a role in disease transmission.
File Format	PDF HTM / HTML
DOI	10.4269/ajtmh.2009.81.258
PubMed reference number	19635880
Journal	Medline
Volume Number	81
Issue Number	2
Alternate Webpage(s)	http://www.ajtmh.org/docserver/fulltext/14761645/81/2/0810258.pdf?accname=guest&checksum=F1FE0046488E6B6FA7F0A75D3E938835&expires=1541111620&id=id
Alternate Webpage(s)	http://www.ajtmh.org/docserver/fulltext/14761645/81/2/0810258.pdf?accname=guest&checksum=E4E89C51EF108A23F0AC9AD87CECEEA0&expires=1542622026&id=id
Alternate Webpage(s)	https://doi.org/10.4269/ajtmh.2009.81.258
Journal	The American journal of tropical medicine and hygiene
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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