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1P109 ファラオニスハロロドプシンの高次構造及び機能に与えるカ ロテノイド結合の影響 Effect of carotenoid binding to structure and function of Natronomonas pharaonis halorhodopsin
| Content Provider | Semantic Scholar |
|---|---|
| Author | Munazah Andrabi Kenji Mizuguchi |
| Copyright Year | 2014 |
| Abstract | Light driven anion pump halorhodopsin (NpHR), which exists on the biomembrnae of N. Pharaonis, forms trimer and binds a carotenoid of bacterioruberin (BR). In this study, the effect to the structure and function of NpHR by binding of the BR were investigated. NpHR obtained from an E.coli expression system had ability to bind the BR tightly and the binding led to the remarkable increase of the thermal stability of the tertiary and quaternary structure of NpHR. These results indicate that the BR binding has the effect to reduce the thermal fluctuation of the NpHR following to the increase of the structural stability. The effect of the BR binding to the anion pump activity of the NpHR is also discussed based on the PatchClamp experiment. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.jstage.jst.go.jp/article/biophys/53/supplement1-2/53_KJ00009360232/_pdf/-char/en |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
