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In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Watanabe, Wataru Matsunaga, Sachihiro Higashi, Tsunehito Fukui, Kiichi Itoh, Kazuyoshi |
| Copyright Year | 2008 |
| Abstract | Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein. |
| Starting Page | 031213 |
| Ending Page | 031213 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1117/1.2939401 |
| Alternate Webpage(s) | https://biomedicaloptics.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.2939401&isResultClick=False |
| Alternate Webpage(s) | https://neurophotonics.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.2939401&isResultClick=False |
| Alternate Webpage(s) | https://www.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.2939401 |
| PubMed reference number | 18601537 |
| Alternate Webpage(s) | https://doi.org/10.1117/1.2939401 |
| Journal | Medline |
| Volume Number | 13 |
| Issue Number | 3 |
| Journal | Journal of biomedical optics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
