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Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Boutrus, Steven Greiner, Cherry Hwu, Derrick Chan, Michael K. Kuperwasser, Charlotte Lin, Charles P. Georgakoudi, Irene |
| Copyright Year | 2007 |
| Abstract | The recent introduction of the in vivo flow cytometer for real-time, noninvasive detection and quantification of cells circulating in the vasculature of small animals has provided a powerful tool for tracking the roles of different types of cells in disease progression. We describe a portable version of the device, which provides the capability to: a) excite and detect fluorescence at two distinct colors simultaneously, and b) perform data analysis in real time. These advances improve significantly the utility of the instrument and provide a means of increasing detection specificity. As examples, we present the depletion kinetics of circulating green fluorescent protein (GFP)-labeled breast cancer cells in the vasculature of mice, and the specific detection of circulating hematopoietic stem cells labeled in vivo with two antibodies. |
| Starting Page | 020507 |
| Ending Page | 020507 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1117/1.2722733 |
| PubMed reference number | 17477705 |
| Journal | Medline |
| Volume Number | 12 |
| Issue Number | 2 |
| Alternate Webpage(s) | https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-12/issue-02/020507/Portable-two-color-in-vivo-flow-cytometer-for-real-time/10.1117/1.2722733.pdf |
| Alternate Webpage(s) | https://www.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.2722733 |
| Alternate Webpage(s) | https://www.spiedigitallibrary.org/journalIssue/Download?DOI=10.1117/1.2722733&downloadType=journal+article&fullDOI=10.1117/1.2722733&isResultClick=True |
| Alternate Webpage(s) | https://doi.org/10.1117/1.2722733 |
| Journal | Journal of biomedical optics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
